ABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Anti-Bacterial Agents/pharmacology , Cefotaxime/analogs & derivatives , Cholangiopancreatography, Endoscopic Retrograde , Gallstones/surgery , Gram-Negative Anaerobic Bacteria/drug effects , Gram-Negative Bacterial Infections/diagnosis , Metronidazole/therapeutic use , Microbial Sensitivity Tests , RNA, Ribosomal, 16S/chemistry , Sequence Analysis, DNA , Tomography, X-Ray ComputedABSTRACT
ABSTRACT This article presents a case of tick infestation of the lower eyelid by a previously unreported species. A 71-year-old male presented with a tick attached to the lower eyelid. The tick was identified morphologically, and then molecularly via polymerase chain reaction (PCR) and sequencing of its DNA. In addition, a review of the literature relevant to the genera of ticks associated with infestation of the human eye is provided. The tick, which was in the nymphal developmental stage, was first identified according to taxonomic keys as Dermacentor sp. For complete species identification, 16s rDNA gene PCR and sequencing were performed, which showed that the tick was D. marginatus. Systematizing tick species could assist physicians in determining the potential for transmission of tick-borne human diseases.
RESUMO Este artigo apresenta um caso de infestação por carrapatos da pálpebra inferior por uma espécie previamente não declarada. Um homem de 71 anos de idade apresentou-se com um carrapato grudado na pálpebra inferior. O carrapato foi identificado morfologicamente, e, em seguida, uma estrutura molecular através de reacção em cadeia da polimerase (PCR) e a sequenciação do seu DNA. Além disso, uma análise da literatura pertinente aos gêneros de carrapatos associados à infestação do olho humano é fornecido. O carrapato, que estava em fase de desenvolvimento das ninfas, foi identificado pela primeira vez de acordo com chaves taxonômicas com o Dermacentor sp. Para identificação de espécies completa, gene 16S rDNA PCR e sequenciamento foram realizadas, que mostrou que o carrapato foi D. marginatus. Sistematizando espécie de carrapato poderia ajudar os médicos a determinar o potencial de transmissão de doenças humanas transmitidas por carrapatos.
Subject(s)
Humans , Male , Aged , Tick Infestations/parasitology , Ticks/classification , Ticks/genetics , Ticks/parasitology , Eye Infections, Parasitic , Eyelids/parasitology , Phylogeny , DNA/isolation & purification , DNA/chemistry , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/chemistry , Polymerase Chain Reaction , Eyelid Diseases/parasitology , Nucleic Acid ConformationABSTRACT
BACKGROUND: Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has recently become the method of choice for identifying bacterial species. The purpose of this study was to evaluate the ability of MALDI-TOF MS (Bruker Daltonics GmbH, Germany) in combination with an improved database to identify various Acinetobacter species. METHODS: A total of 729 Acinetobacter clinical isolates were investigated, including 447 A. baumannii, 146 A. nosocomialis, 78 A. pittii, 18 A. ursingii, 9 A. bereziniae, 9 A. soli, 4 A. johnsonii, 4 A. radioresistens, 3 A. gyllenbergii, 3 A. haemolyticus, 2 A. lwoffii, 2 A. junii, 2 A. venetianus, and 2 A. genomospecies 14TU. After 212 isolates were tested with the default Bruker database, the profiles of 63 additional Acinetobacter strains were added to the default database, and 517 isolates from 32 hospitals were assayed for validation. All strains in this study were confirmed by rpoB sequencing. RESULTS: The addition of the 63 Acinetobacter strains' profiles to the default Bruker database increased the overall concordance rate between MALDI-TOF MS and rpoB sequencing from 69.8% (148/212) to 100.0% (517/517). Moreover, after library modification, all previously mismatched 64 Acinetobacter strains were correctly identified. CONCLUSIONS: MALDI-TOF MS enables the prompt and accurate identification of clinically significant Acinetobacter species when used with the improved database.
Subject(s)
Humans , Acinetobacter Infections/microbiology , Acinetobacter baumannii/chemistry , Bacterial Proteins/chemistry , Databases, Factual , Phylogeny , RNA, Ribosomal, 16S/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
No abstract available.
Subject(s)
Adult , Aged , Humans , Male , Middle Aged , Young Adult , Biopsy , Colon/microbiology , Fusobacterium/genetics , Inflammatory Bowel Diseases/microbiology , Polymerase Chain Reaction , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, DNAABSTRACT
No abstract available.
Subject(s)
Animals , Dogs , Female , Humans , Middle Aged , Anti-Bacterial Agents/pharmacology , Bites and Stings , Disk Diffusion Antimicrobial Tests , Pasteurella/drug effects , Pasteurella Infections/diagnosis , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, DNA , Soft Tissue Infections/diagnosisABSTRACT
BACKGROUND: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that included bacteria and other microorganisms. The NGS results were compared with the results of other assays, and NGS was evaluated for its feasibility for predicting vaginitis. METHODS: In total, 89 vaginal swab specimens were collected. Microscopic examinations of Gram staining and microbiological cultures were conducted on 67 specimens. NGS was performed with GS junior system on all of the vaginal specimens for the 16S rRNA, internal transcribed spacer (ITS), and Tvk genes to detect bacteria, fungi, and Trichomonas vaginalis. In addition, DNA probe assays of the Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis were performed. Various predictors of diversity that were obtained from the NGS data were analyzed to predict vaginitis. RESULTS: ITS sequences were obtained in most of the specimens (56.2%). The compositions of the intermediate and vaginitis Nugent score groups were similar to each other but differed from the composition of the normal score group. The fraction of the Lactobacillus spp. showed the highest area under the curve value (0.8559) in ROC curve analysis. The NGS and DNA probe assay results showed good agreement (range, 86.2-89.7%). CONCLUSIONS: Fungi as well as bacteria should be considered for the investigation of vaginal microbiome. The intermediate and vaginitis Nugent score groups were indistinguishable in NGS. NGS is a promising diagnostic tool of the vaginal microbiome and vaginitis, although some problems need to be resolved.
Subject(s)
Female , Humans , Area Under Curve , Bacteria/genetics , Bacterial Proteins/genetics , Candida/genetics , Fungal Proteins/genetics , Gardnerella vaginalis/genetics , High-Throughput Nucleotide Sequencing , Microbiota , RNA, Ribosomal, 16S/chemistry , ROC Curve , Sequence Analysis, DNA , Trichomonas vaginalis/genetics , Vagina/microbiology , Vaginitis/diagnosisABSTRACT
Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Anti-Bacterial Agents/pharmacology , DNA, Bacterial/chemistry , Drug Resistance, Bacterial/drug effects , Linezolid/pharmacology , Microbial Sensitivity Tests , Oxacillin/pharmacology , Phenotype , RNA, Ribosomal, 16S/chemistry , Sequence Analysis, DNA , Staphylococcal Infections/drug therapy , Staphylococcus/drug effects , Vancomycin/pharmacologyABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Asian People , Enterobacteriaceae/classification , Phylogeny , Pneumonia/diagnosis , Pulmonary Disease, Chronic Obstructive/diagnosis , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, DNAABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Bronchiectasis/diagnosis , Mycobacterium/classification , Mycobacterium Infections/diagnosis , Phylogeny , Polymerase Chain Reaction , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, DNAABSTRACT
No abstract available.
Subject(s)
Humans , Male , Middle Aged , Asian People , Flavobacteriaceae Infections , Flavobacterium/genetics , Liver Cirrhosis, Alcoholic/blood , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, DNAABSTRACT
No abstract available.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Infant, Newborn , Male , Middle Aged , Anti-Bacterial Agents/pharmacology , Ceftriaxone/pharmacology , Echocardiography , Endocarditis, Bacterial/diagnosis , Immunosuppressive Agents/therapeutic use , Microbial Sensitivity Tests , Micrococcaceae/drug effects , RNA, Ribosomal, 16S/chemistry , Republic of KoreaABSTRACT
No abstract available.
Subject(s)
Female , Humans , Middle Aged , Anti-Bacterial Agents/pharmacology , DNA, Bacterial/analysis , Hypertrophy/diagnosis , Magnetic Resonance Imaging , Microbial Sensitivity Tests , Mycobacterium/classification , Nontuberculous Mycobacteria/classification , Phylogeny , RNA, Ribosomal, 16S/chemistry , Real-Time Polymerase Chain Reaction , Republic of Korea , Sequence Analysis, RNA , Sequence Homology, Nucleic Acid , Tenosynovitis/diagnosisABSTRACT
No abstract available.
Subject(s)
Adult , Humans , Male , Anti-Bacterial Agents/pharmacology , Bone Marrow Transplantation , Capnocytophaga/drug effects , Gram-Negative Bacterial Infections/complications , Leukemia, Lymphocytic, Chronic, B-Cell/complications , Microbial Sensitivity Tests , Penicillanic Acid/analogs & derivatives , Piperacillin/pharmacology , RNA, Ribosomal, 16S/chemistry , Sequence Analysis, RNA , Sequence Homology, Nucleic Acid , Transplantation, Homologous , Treatment OutcomeABSTRACT
No abstract available.
Subject(s)
Humans , Male , Middle Aged , Asian People , Bacteremia/complications , Base Sequence , Diabetes Mellitus, Type 2/complications , Diabetic Foot/microbiology , Gram-Positive Cocci/genetics , RNA, Ribosomal, 16S/chemistry , Republic of KoreaABSTRACT
BACKGROUND: Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One platform that has recently become available is the Kiestra Total Laboratory Automation (TLA) system (BD Kiestra B.V., the Netherlands). This fully automated sample processing system, equipped with digital imaging technology, allows superior detection of microbial growth. Combining this approach with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) (Bruker Daltonik, Germany) is expected to enable more rapid identification of pathogens. METHODS: Early growth detection by digital imaging using Kiestra TLA combined with MS was compared to conventional methods (CM) of detection. Accuracy and time taken for microbial identification were evaluated for the two methods in 219 clinical blood culture isolates. The possible clinical impact of earlier microbial identification was assessed according to antibiotic treatment prescription. RESULTS: Pathogen identification using Kiestra TLA combined with MS resulted in a 30.6 hr time gain per isolate compared to CM. Pathogens were successfully identified in 98.4% (249/253) of all tested isolates. Early microbial identification without susceptibility testing led to an adjustment of antibiotic regimen in 12% (24/200) of patients. CONCLUSIONS: The requisite 24 hr incubation time for microbial pathogens to reach sufficient growth for susceptibility testing and identification would be shortened by the implementation of Kiestra TLA in combination with MS, compared to the use of CM. Not only can this method optimize workflow and reduce costs, but it can allow potentially life-saving switches in antibiotic regimen to be initiated sooner.
Subject(s)
Humans , Automation, Laboratory , Candida albicans/genetics , Disk Diffusion Antimicrobial Tests , Gram-Negative Bacteria/genetics , Gram-Positive Bacteria/genetics , RNA, Ribosomal, 16S/chemistry , Retrospective Studies , Sequence Analysis, RNA , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
No abstract available.
Subject(s)
Humans , Male , Middle Aged , Base Sequence , Carnobacteriaceae/genetics , Databases, Genetic , Gram-Positive Bacterial Infections/blood , Polymerase Chain Reaction , RNA, Ribosomal, 16S/chemistry , Sequence Analysis, RNAABSTRACT
No abstract available.
Subject(s)
Humans , Male , Middle Aged , Actinobacteria/genetics , Bacteremia/diagnosis , Polymerase Chain Reaction , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Sequence Analysis, RNA , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Anti-Bacterial Agents/therapeutic use , Bursitis/diagnosis , Cefoperazone/therapeutic use , Diabetes Mellitus, Type 2/complications , Nocardia/genetics , Polymerase Chain Reaction , RNA, Ribosomal, 16S/chemistry , Sequence Analysis, RNA , Sulbactam/therapeutic useABSTRACT
No abstract available.
Subject(s)
Aged , Humans , Male , Ampicillin/therapeutic use , Anti-Bacterial Agents/therapeutic use , Antimetabolites, Antineoplastic/therapeutic use , Asian People , Cytarabine/therapeutic use , Drug Therapy, Combination , Idarubicin/therapeutic use , Leukemia, Myeloid, Acute/complications , Moraxella/genetics , Moraxellaceae Infections/diagnosis , RNA, Ribosomal, 16S/chemistry , Republic of Korea , Respiratory Tract Infections/diagnosis , Sequence Analysis, RNA , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Sulbactam/therapeutic useABSTRACT
Clostridium difficile, an anaerobic, spore-forming, gram-positive, rod-shaped bacterium, is the most common nosocomial pathogen causing pseudomembranous colitis. C. difficile is not intrinsically invasive and rarely infects extraintestinal sites. The bacterium, therefore, is not commonly detected in blood cultures. Here, we report a case of C. difficile bacteremia in a patient who had underwent loop ileostomy because of rectal obstruction following metastatic colon cancer originated from prostate cancer.